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pluriSelect—让阳选后的细胞与磁珠Say goodbye!

作者:admin信息来源:达科为日期:2019年04月28日打印字体:  
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回顾上期Cell Strainer-细胞过滤和纯化的好帮手pluriSelect公司不同孔径规格的细胞筛网Mini StrainerpluriStrainer深受大家的青睐。

小达君化身大白给细胞分选加些料,助力大家的分选实验。今天介绍pluriSelect的一款分选的利器,不用千篇的内容就能掌握细胞分选的新思路, pluriSelect支招来搞定细胞阳选后的磁珠!

阳选后细胞携带的磁珠也可以去掉,这是个神马操作呢?这么好的idea,全血分选当然不在话下,并且分选后血小板或红细胞的污染非常低,得到的细胞也可以用于下游另一种细胞的阳性或阴性富集实验。可分选的样本类型也包括 PBMC, Buffy Coat, Cord Blood, Bone Marrow,Primary Cell Solution.

分选原理如下:

人的分选的指标如下(规格均为2ml装):

货号

产品名称

19-00200-10

CD2 S-pluriBead®  anti-hu, 2ml

19-00300-10

CD3 S-pluriBead®  anti-hu, 2ml

19-00400-10

CD4 S-pluriBead®  anti-hu, 2ml

19-00500-10

CD5 S-pluriBead®  anti-hu, 2ml

19-00700-10

CD7 S-pluriBead®  anti-hu, 2ml

19-00800-10

CD8 S-pluriBead®  anti-hu, 2ml

19-00900-10

CD9 S-pluriBead®  anti-hu, 2ml

19-011a0-10

CD11a S-pluriBead®  anti-hu, 2ml

19-011b0-10

CD11b S-pluriBead®  anti-hu, 2ml

19-01400-10

CD14 S-pluriBead®  anti-hu, 2ml

19-01500-10

CD15 S-pluriBead®  anti-hu, 2ml

19-01600-10

CD16 S-pluriBead®  anti-hu, 2ml

19-01900-10

CD19 S-pluriBead®  anti-hu, 2ml

19-02100-10

CD21 S-pluriBead®  anti-hu, 2ml

19-03100-10

CD31 S-pluriBead®  anti-hu, 2ml

19-04300-10

CD43 S-pluriBead®  anti-hu, 2ml

19-04400-10

CD44 S-pluriBead®  anti-hu, 2ml

19-04500-10

CD45 S-pluriBead®  anti-hu, 2ml

19-04700-10

CD47 S-pluriBead®  anti-hu, 2ml

19-04300-10

CD43 S-pluriBead®  anti-hu, 2ml

19-04400-10

CD44 S-pluriBead®  anti-hu, 2ml

19-04400-20

CD44 M-pluriBead®  anti-hu, 2ml

19-04500-10

CD45 S-pluriBead®  anti-hu, 2ml

19-04700-10

CD47 S-pluriBead®  anti-hu, 2ml

19-05500-10

CD55 S-pluriBead®  anti-hu, 2ml

19-09500-10

CD95 S-pluriBead®  anti-hu, 2ml

19-11700-10

CD117 S-pluriBead®  anti-hu, 2ml

19-13800-10

CD138 S-pluriBead®  anti-hu, 2ml

19-19300-10

CD193 S-pluriBead®  anti-hu, 2ml

19-235a0-10

CD235a S-pluriBead®  anti-hu, 2ml

19-27100-10

CD271 S-pluriBead®  anti-hu, 2ml

大鼠、小鼠、猪、羊、牛、狗、马、兔子的分选指标如下(规格均为2ml装)

货号

产品名称

22-00400-10

CD4  S-pluriBead® anti-rat,  2ml

22-008b0-10

CD8b  S-pluriBead® anti-rat,  2ml

22-011b0-10

CD11b  S-pluriBead® anti-rat,  2ml

22-04400-10

CD44  S-pluriBead® anti-rat,  2ml

22-04500-10

CD45  S-pluriBead® anti-rat,  2ml

22-05500-10

CD55  S-pluriBead® anti-rat,  2ml

22-TCRAB-10

Anti TCRAB  S-pluriBead® anti-rat,  2ml

23-011b0-10

CD11b  S-pluriBead®  anti-porcine, 2ml

23-01400-10

CD14  S-pluriBead®  anti-porcine, 2ml

23-02100-10

CD21  S-pluriBead®  anti-porcine, 2ml

23-02900-10

CD29  S-pluriBead®  anti-porcine, 2ml

23-04400-10

CD44  S-pluriBead®  anti-porcine, 2ml

23-04700-10

CD47  S-pluriBead®  anti-porcine, 2ml

23-062L0-10

CD62L  S-pluriBead®  anti-porcine, 2ml

23-09000-10

CD90  S-pluriBead®  anti-porcine, 2ml

23-HLARP-10

HLARP  S-pluriBead®  anti-porcine, 2ml

23-RBC00-10

RBC  S-pluriBead®  anti-porcine, 2ml

24-02900-10

CD29  S-pluriBead® anti-sheep,  2ml

24-04100-10

CD41/61  S-pluriBead® anti-sheep,  2ml

24-04400-10

CD44  S-pluriBead® anti-sheep,  2ml

24-04500-10

CD45  S-pluriBead® anti-sheep,  2ml

24-04700-10

CD47  S-pluriBead® anti-sheep,  2ml

24-062L0-10

CD62L  S-pluriBead® anti-sheep,  2ml

24-27100-10

CD271  S-pluriBead® anti-sheep,  2ml

24-HLARP-10

HLARP  S-pluriBead® anti-sheep,  2ml

25-00900-10

CD9  S-pluriBead®  anti-bovine, 2ml

25-01400-10

CD14  S-pluriBead®  anti-bovine, 2ml

25-02100-10

CD21  S-pluriBead®  anti-bovine, 2ml

25-045R0-10

CD45R  S-pluriBead®  anti-bovine, 2ml

25-04700-10

CD47  S-pluriBead®  anti-bovine, 2ml

25-062L0-10

CD62L  S-pluriBead®  anti-bovine, 2ml

25-11700-10

CD117  S-pluriBead®  anti-bovine, 2ml

25-HLARP-10

HLARP  S-pluriBead®  anti-bovine, 2ml

27-00400-10

CD4  S-pluriBead®  anti-canine, 2ml

27-02900-10

CD29  S-pluriBead®  anti-canine, 2ml

29-00300-10

CD3  S-pluriBead® anti-ms,  2ml

29-00400-10

CD4  S-pluriBead® anti-ms,  2ml

29-00800-10

CD8  S-pluriBead® anti-ms,  2ml

29-011b0-10

CD11b  S-pluriBead® anti-ms,  2ml

29-011c0-10

CD11c  S-pluriBead® anti-ms,  2ml

29-01400-10

CD14  S-pluriBead® anti-ms,  2ml

29-01900-10

CD19  S-pluriBead® anti-ms,  2ml

29-03100-10

CD31  S-pluriBead® anti-ms,  2ml

29-04500-10

CD45  S-pluriBead® anti-ms,  2ml

29-32600-10

CD326  S-pluriBead® anti-ms,  2ml

29-F4800-10

F4/80  S-pluriBead® anti-ms,  2ml

29-Ly6G0-10

Ly6G  S-pluriBead® anti-ms,  2ml

31-GTaHS-10

universal S-pluriBead® anti-hamster, 2ml

31-GTaMS-10

universal  S-pluriBead® anti-ms,  2ml

31-GTaRB-10

universal  S-pluriBead®  anti-rabbit, 2ml

31-GTaRT-10

universal  S-pluriBead® anti-rat,  2ml

31-RBaGT-10

universal  S-pluriBead® anti-goat,  2ml

31-RBaHU-10

universal  S-pluriBead® anti-hu,  2ml

32-011a0-10

CD11a  S-pluriBead® anti-horse,  2ml

32-04100-10

CD41/61  S-pluriBead® anti-horse,  2ml

33-01400-10

CD14  S-pluriBead®  anti-rabbit, 2ml

33-08100-10

CD81  S-pluriBead®  anti-rabbit, 2ml

不同来源的样本的分选体系:

样本类型

分选体系

Whole Blood

40 µl S-pluriBead® suspension per 1 ml

Other samples

40 µl S-pluriBead® suspension per 1x10targets

Alternatively

40 µl S-pluriBead® suspension per 1x106 total cells

完成分选实验需要搭配如下的辅助试剂:

货号

产品名称

50-01010-80

pluriPlix(用于样本混匀孵育

70-50010-11

S-pluriBead®  Mini Reagent Kit(Kit里的各成分可单独订购

70-50010-12

S-pluriBead®  Maxi Reagent Kit(Kit里的各成分可单独订购

有木有发现自己的实验中需要分选的指标呢?是不是对该分选方案充满好奇呢,心动不如行动,那就联系我们下单吧!

下一期我们会推出更精彩的内容,敬请期待!

*具体实验步骤请点击:”步骤“

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